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Exercising "Cultured" Myotubes!

update:2018-12-13
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Features
We succeeded in generating highly developed cultured C2C12 myotubes by manipulating intracellular Ca2+ transients with electric pulse stimulation (EPS), that are endowed with similar properties to in vivo skeletal muscle in terms of (1) excitation-induced contractile activity as a result of de novo sarcomere formation, (2) higher energy expenditure (as assessed by AMPK activation), and (3) improved insulin responsiveness (as assessed by exofacial myc-GLUT4 translocation assay).

Targeted Application(s)/Industry
Taking advantage of our “in vitro Exercise Model", our innovation will be an excellent alternative for the animal experimentation that can be applicable for a wide array of skeletal muscle research including drug screen.

Researchers

Graduate School of Biomedical Engineering

KANZAKI, Makoto , Associate Professor
PhD

Keywords

Related Information

US7829334 Cultured Muscle Cells with High Metabolic Activity and Methods for Production of the Cultured Muscle Cells


Regulatory Mode Shift of Tbc1d1 is Required for insulin-responsive GLUT4 Trafficking Activity (2013) Mol. Biol. Cell 24(6): 809-817.
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