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  • Reserach on Cell Surface Lectin of Lactic Acid Bacteria and their Interaction with Human Colon Mucin

Reserach on Cell Surface Lectin of Lactic Acid Bacteria and their Interaction with Human Colon Mucin

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We first proposed the selection method (screening method) for probiotics such as lactic acid bacteria (LAB) and/or Bifidobacteria to human colon mucin by using a biosensor Biacore. We first discovered LAB with high adhesion to human ABO blood type antigen. We also discovered several adhesin such as surface layer protein, GAPDH, transporter protein, etc.

Targeted Application(s)/Industry
We are expecting the exclusion of harmful bacteria which may be the cause of UC and CD of IBD by using such LAB in the near future.


Graduate School of Agricultural Science

SAITO, Tadao , Professor


Related Information

I-N, Huang et al., New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay. [J. Appl. Microbiol., 114, 854-860, 2012]

M. Watanabe et al., Identification of a new adhesin-like protein from Lactobacillus mucosae ME340 with specific affinity to the human blood group A and B antigens. [J. Appl. Microbiol., 109, 927-935, 2010]

H, Kinoshita et al., Cell surface Lactobacillus plantarum LA318 glycelaldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin.[J. Appl. Mictobiol., 104, 1667-1674, 2008]

H. Uchida et al., Lactobacilli binding human A-antigen expressed in intestinal mucosa. [Res. Microbiol., 157, 659-665, 2006]

H. Uchida et al., Lactic acid bacteria (LAB) bind to human B- or H-antigen expressed on intestinal mucosa. [Biosci. Biotechno. Biochem., 70, 3073-3076, 2006]

H. Uchida et al., A New assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucin[Biosci. Biotechnol. Biochem., 68, 1004-1010, 2004]

T. Saito, Selection of useful probiotic lactic acid bacteria from the Lactobacillus acidophilus group and their applications to functional foods.[Animal. Sci. J., 75, 1-13, 2004]