Research Profile #103 New High-Throughput, High Resolution DNA Methylation Analysis Method, PCR-Luminex for the Diagnosis of Genomic Imprinting Disorders in ART Babies

New High-Throughput, High Resolution DNA Methylation Analysis Method, PCR-Luminex for the Diagnosis of Genomic Imprinting Disorders in ART Babies

update：2020-06-16

Features

The parent-of–origin specific expression of the imprinted genes relies on DNA methylation acquired during gametogenesis. Recent studies indicate the possibility of excess imprinting disease occurrence in babies conceived by assisted reproductive technologies (ART). PCR-Luminex method is powerful tool for the methylation analyses, suitable for the use at the clinical diagnosis level because of taking much time and multistep procedures.

Targeted Application(s)/Industry

We have developed a new high-throughput, high resolution DNA methylation analysis method. PCT international patent was performed.

Researchers

Environment and Genome Research Center, Graduate School of Medicine

ARIMA Takahiro , Professor
Medical Doctor

Laboratory Website

researchmap

Keywords

Related Information

Kobayashi H, Sato A, Otsu E, Hiura H, Tomatsu C, Utsunomiya T, Sasaki H, Yaegashi N, Arima T. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet. 16, 2542-2551, 2007.

Sato A, Hiura H, Okae H, Miyauchi N, Abe Y, Utsunomiya T, Yaegashi N, Arima T. Assessing loss of imprint methylation in sperm from subfertile men using novel methylation PCR-Luminex analysis. Fertility and Sterility. 95: 129-34 2011.

Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T. High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method. BMC Medical Genomics. 5, 8-17, 2012.