Development of Production System of Valuable Proteins Using a Koji-Mold, Aspergillus Oryzae, as a Host
update：2020-06-16
Features
Because a koji-mold, Aspergillus oryzae, has an ability to secret a higher amount of proteins and is regarded as a safe microorganism, it has been expected as a promising host for production of useful proteins derived from plants and animals. However, Aspergillus oryzae produces various proteolytic enzymes that could degrade heterologous proteins produced, and hence we constructed mutant strains in which a transcription factor gene involved in proteolytic enzyme production was disrupted. In addition, codon optimization of heterologous gene resulted in a significant increase in the stability of heterologous gene transcripts. These attempts would lead to an increase in the amount of valuable proteins produced.Targeted Application(s)/Industry
The technology could be applied to the high-level production of pharmaceutical proteins and industrially useful enzymes. We look forward to developing the collaborative project with enzyme and pharmaceutical industries.Researchers
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