Exercising "Cultured" Myotubes!
update：2020-06-16
Features
We succeeded in generating highly developed cultured C2C12 myotubes by manipulating intracellular Ca2+ transients with electric pulse stimulation (EPS), that are endowed with similar properties to in vivo skeletal muscle in terms of (1) excitation-induced contractile activity as a result of de novo sarcomere formation, (2) higher energy expenditure (as assessed by AMPK activation), and (3) improved insulin responsiveness (as assessed by exofacial myc-GLUT4 translocation assay).Targeted Application(s)/Industry
Taking advantage of our “in vitro Exercise Model", our innovation will be an excellent alternative for the animal experimentation that can be applicable for a wide array of skeletal muscle research including drug screen.Researchers
Graduate School of Biomedical Engineering
KANZAKI Makoto
, Professor
PhD
Keywords
Related Information
Regulatory Mode Shift of Tbc1d1 is Required for insulin-responsive GLUT4 Trafficking Activity (2013) Mol. Biol. Cell 24(6): 809-817.
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